Injected-Ion Mobility Analysis of Biomolecules

نویسندگان

  • Yansheng Liu
  • Stephen J. Valentine
  • Anne E. Counterman
چکیده

This Report describes biomolecular structural studies using the injected-ion mobility/MS approach. Several examples will illustrate how these methods can be used to resolve and characterize conformations, examine the dynamics of protein unfolding and folding in the gas phase, and complement MS methods for characterizing oligosaccharide isomers. [The development and application of these methods has been reviewed elsewhere (6).]

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Three-dimensional ion mobility/TOFMS analysis of electrosprayed biomolecules.

An ion mobility/mass spectrometry technique has been developed to record mass-resolved ion mobility distributions for multiple ions simultaneously. The approach involves a new instrument that couples an electrospray ion source to an injected-ion drift tube/time-of-flight mass spectrometer. Individual components in a mixture of ions are separated by mobility differences in a drift tube and subse...

متن کامل

DETECTION AND MEASUREMENT OF ACETONE IN THE BREATH OF DIABETICS BY ION MOBILITY SPECTROMETRY METHOD

Background: The ion mobility spectrometry (IMS) is an analytical technique that is widely used due to its high sensitivity and speed for the detection of ionized molecules in gas phase and under atmospheric pressure. Breath analysis is a new method for obtaining information about person's clinical conditions that is considered by researchers. Human exhaled air contains a variety of components s...

متن کامل

PMM - 111 Nanoelectrospray Differential Ion Mobility Spectrometry for Protein Sizing and Molecular Mass Determination: Method Development and Validation

The characterization of large biomolecules, including the determination of their size and molecular mass (Mr), is important for understanding fundamental aspects of their biochemical behavior. Even though MS has dominated this type of analysis, another similar technique based on nanoelectrospray ion mobility spectrometry, referred to as gas phase electrophoretic mobility molecule analysis (GEMM...

متن کامل

Application of Ion Mobility Spectrometry for Determination of Morphine in Human Urine

In this study, a rapid, simple and sensitive ion mobility spectrometry (IMS) method with corona discharge as ionization source was described for the morphine determination in human urine. Morphine was extracted and purified from urine samples using solid phase extraction procedure with C18 column. It can offer the clean extracts which no extra peaks were observed in IMS. Under operating experim...

متن کامل

Towards the Analysis of High Molecular Weight Proteins and Protein complexes using TIMS-MS.

In the present work, we demonstrate the potential and versatility of TIMS for the analysis of proteins, DNA-protein complexes and protein-protein complexes in their native and denatured states. In addition, we show that accurate CCS measurement are possible and in good agreement with previously reported CCS values using other IMS analyzers (<5% difference). The main challenges for the analysis ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2007